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| 2. |  | JUENGEL, J. L.; MELNER, M. H.; CLAPPER, J. A.; TURZILLO, A. M.; MOSS, G. E.; NETT, T. M.; NISWENDER, G. D. Steady-state concentrations of mRNA encoding two inhibitors of protein kinase C in ovine luteal tissue. Journal of Reproduction and Fertility, v. 113, n. 2, p. 299-305, 1998.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/07/2009 |
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Data da última atualização: |
14/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W. |
|
Afiliação: |
Karen Sumire Kubo, APTA; R. M. Stuart, APTA; Juliana Freitas-Ástua, CNPMF; Renata Antonioli-Luizon, APTA; Eliane Cristina Locali-Fabris, APTA; Helvecio Della Coletta-Filho, APTA; Marcos Antonio Machado, APTA; Elliot Watanabe Kitajima, ESALQ. |
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Título: |
Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009. |
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ISSN: |
0304-8608 |
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DOI: |
DOI 10.1007/s00705-009-0395-8 |
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Idioma: |
Inglês |
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Conteúdo: |
The variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of 18 genera of orchids were collected from Brazil, Costa Rica and Australia. The SSCP analysis yielded six different band patterns, and phylogenetic analysis based on the nucleotide fragment sequence obtained in this work and six available in GenBank showed two different groups, one with isolates 023Germany and So-Japan, and other with the rest of the isolates. None of the analyses showed geographic correlation among the Brazilian strains. The data obtained in this study showed a low genetic variation in this region of the genome; the dN/dS ratio of 0.251-0.405 demonstrated a negative selective pressure that maintains the stability of the analyzed fragments. |
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Palavras-Chave: |
Nuclear type. |
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Thesaurus NAL: |
Orchid fleck virus. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 01736naa a2200253 a 4500
001 1654417
005 2023-06-14
008 2009 bl uuuu u00u1 u #d
022 $a0304-8608
024 7 $aDOI 10.1007/s00705-009-0395-8$2DOI
100 1 $aKUBO, K. S.
245 $aEvaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid.$h[electronic resource]
260 $c2009
520 $aThe variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of 18 genera of orchids were collected from Brazil, Costa Rica and Australia. The SSCP analysis yielded six different band patterns, and phylogenetic analysis based on the nucleotide fragment sequence obtained in this work and six available in GenBank showed two different groups, one with isolates 023Germany and So-Japan, and other with the rest of the isolates. None of the analyses showed geographic correlation among the Brazilian strains. The data obtained in this study showed a low genetic variation in this region of the genome; the dN/dS ratio of 0.251-0.405 demonstrated a negative selective pressure that maintains the stability of the analyzed fragments.
650 $aOrchid fleck virus
653 $aNuclear type
700 1 $aSTUART, R. M.
700 1 $aFREITAS-ASTÚA, J.
700 1 $aANTONIOLI-LUIZON, R.
700 1 $aLOCALI-FABRIS, E. C.
700 1 $aCOLETTA-FILHO, H. D.
700 1 $aMACHADO, M. A.
700 1 $aKITAJIMA, E. W.
773 $tArchives of Virology$gv. 154, n. 6, p. 1009-1014, mai. 2009.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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